O O OH OH OH N+ GE Healthcare Multimodal media Capto adhere Data file. The most pronounced are ionic interaction, hydrogen bonding, and hydrophobic interaction. The Capto adhere ligand, N-benzyl-N-methylethanolamine, exhibits many functionalities for interaction. Develop a solid control strategy to obtain a. Get critical insights on resin variation within ligand density specification interval. The kit allows you to study the potential impact that resin ligand density might have on the process outcome. These resins offer a different selectivity than the traditional ion exchange and hydrophobic interaction chromatography and are likely to make inroads into many process platforms over time as users create new applications around them. The multimodal ligand structure for Capto adhere is shown in Fig 2. The Process Characterization Kit is a tool for gaining deeper chromatography process understanding. This resin can also be used as a step for capture step. It can be operated entirely based on pH or salt with slight change in pH. As in HCIC, protein binding can take place at relatively less salt concentration, thus offering an alternative to HIC where high salt requirement for binding is a cumbersome operation. This desorption can be further enhanced by decreasing hydrophobicity. Protein desorption is prompted by electrostatic charge repulsion by decreasing the pH. Binding of protein is based on mild hydrophobic interaction and conducted at near-neutral pH condition where the pyridine group of the ligand is uncharged. HCIC is based on pH-dependent behavior of ionizable dual-mode ligand. In another example MEPHyperCel (Pall Corp., East Hills, NY) resin operates via mixed-mode interactions governed by hydrophobic charge-induction chromatography (HCIC). It can be operated as anion exchanger as well as hydrophobic mode by applying suitable process condition. One such example is Capto Adhere (Cytiva, Uppsala, Sweden). Mixed-mode resins based on ion exchange along with multimodal interactions can offer single-step operation as an alternative to two-step process of ion exchange and HIC. Traditionally, hydroxyapatite has been used for separation of protein therapeutics from host and media proteins, aggregates, DNA, and Protein A, all of which tend to bind more tightly. Mixed-mode resins based on hydroxyapatite, made from calcium phosphate, have both positive and negative charges and interact with proteins through a combination of electrostatic interactions and coordination complex formation. This can result in a different selectivity than what is offered by traditional ion-exchange or hydrophobic interaction chromatography, thus offering a larger range of ionic strength and pH under which significant separation can occur (6,7). These resins combine different types of interactions such as ionic interaction, hydrogen bonding, and hydrophobic interaction. Recently, mixed-mode, ion-exchange ligands with enhanced binding strength for aggregates through the addition of hydrophobic functionality have been brought to market.
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